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KMID : 1007520130220010289
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Food Science and Biotechnology
2013 Volume.22 No. 1 p.289 ~ p.293
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Direct Fermentation of Starch to L(+)-Lactic Acid by Fed-batch Culture of Lactobacillus manihotivorans
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Son Min-Shik
Kwon Yun-Joong
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Abstract
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The ability of Lactobacillus manihotivorans LMG 18010 to produce L(+)-lactic acid from a high concentration of starch by direct fermentation was investigated. To improve the production of lactic acid from a highly concentrated starch, fed-batch cultures were carried out with different feeding strategies to reduce substrate inhibition. The highest lactic acid production was 71.4 g/L, which was obtained by intermittent feeding of concentrated starch media containing nitrogen sources. After 5 days of fermentation, about 87% of the substrate was consumed with a volumetric productivity of 0.6 g/L·h which is 1.8 times higher than that obtained from batch fermentation. This result clearly shows that intermittant feeding of a highly concentrated substrate solution during fermentation is an efficient way of eliminating substrate inhibition.
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KEYWORD
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starch, L(+)-lactic acid, direct fermentation, Lactobacillus manihotivorans
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